Agilent Technologies, Inc. - Diagnostics and Genomics Kit,QuikChange II site-directed 10 rxn
The QuikChange II Site-Directed Mutagenesis Kit (10 reactions) from Agilent Technologies enables efficient and reliable introduction of point mutations, insertions, or deletions into double-stranded plasmid DNA. Utilizing a simple PCR-based method, the kit allows for site-specific mutagenesis without the need for subcloning or single-stranded DNA. The kit includes PfuUltra high-fidelity DNA polymerase, dNTP mix, control plasmid, and primers, providing all necessary reagents for successful mutagenesis. The protocol is optimized for high mutation efficiency and minimal background, making it suitable for a wide range of molecular biology applications.